Column: Biotechnology Today - Chromatography Online
Column: Biotechnology Today
Ira Krull, editor of 'Biotechnology Today', is an associate professor of chemistry at Northeastern University, Boston, Massachusetts, USA, and a member of LCGC's editorial advisory board. Co-editor Anurag Rathore is a consultant and associate professor with the Indian Institute of Delhi, India.

The Role of Elution Gradient Shape in the Separation of Protein Therapeutics

September 1, 2014

A discussion of the role of the gradient in separation by reversed-phase and ion-exchange HPLC, with data from two products to illustrate the key points

HILIC and Its Applications for Biotechnology, Part II

January 14, 2014

The modern usage and specific applications of HILIC for two other major classes of analytes — glycopeptides and glycoproteins — are discussed.

HILIC and Its Applications for Biotechnology, Part I

December 1, 2013

Answers to common questions about HILIC, and an overview of its application to biopharmaceutical analysis.

A Rapid HPLC Method for Enabling PAT Application for Processing of GCSF

November 1, 2013

The use of a monolithic column reduces analysis time in an on-line biopharmaceutical HPLC analysis of granulocyte colony stimulating factor.

Analytical Tools for Enabling Process Analytical Technology: HPLC and Quantitative Polymerase Chain Reaction

January 1, 2013

How to use these tools to develop an efficient and robust process

The Use of Light-Scattering Detection with SEC and HPLC for Protein and Antibody Studies, Part II: Examples and Comparison to Mass Spectrometry

December 1, 2012

Detailed examples of light-scattering techniques are presented as well as how their performance compares to MS and other methods.

The Use of Light-Scattering Detection with SEC and HPLC for Protein and Antibody Studies, Part I: Background, Theory, and Potential Uses

September 1, 2012

Light-scattering detection: the theory and the advantages

Bioanalytical Tools for the Characterization of Biologics and Biosimilars

May 1, 2012

LC–MS and CE–MS are the go-to techniques for characterizing biologics, but often help is needed from a range of other methods.

Analytical Tools for Enabling Process Analytical Technology (PAT) Applications in Biotechnology

January 1, 2012

For PAT, you need to choose the right analytical methods. Here's how.


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